Hauptaufgaben

• Projektleitung
• Themenbereichsleitung: Metabolomics, Medizinische Analytik
• Leitung Massenspektrometrie
• Etablierung und Entwicklung neuer analytischer Methoden am Institut

Werdegang

• 1993-2000 Diplomstudium Chemie an der Universität Graz
• 1998 - 1999 Mitarbeiter bei Hygienicum, Institut für Mikrobiologie und Hygieneconsulting (Bearbeitung von Projekten, Leitung eines Fachbereiches)
• 2001 – 2002 Wissenschaftlicher Mitarbeiter, Diabetesambulanz Univ. Kinik Graz
• Seit Juli 2002 Mitarbeiter am Institut für Medizinische Systemtechnik und Gesundheitsmanagement.
• 2001-2005 Dissertation an der Karl Franzens Universität Graz, Institut für Moleulabiologie, Biochemie und Mikrobiologie in Kooperation mit Joanneum Research. Titel: „Development of New Analytical Methods in Diabetes Research:  Application Potential of Chromatographic Separation Techniques Coupled with Mass Spectrometry“

Interessensgebiete

Massenspektrometrie, Metabolomics, Stoffwechselforschung

Publikationen

Journal Article

Tobias Eisenberg, Heide Knauer, Alexandra Schauer, Sabrina Büttner, Christoph Ruckenstuhl, Didac Carmona-Gutierrez, Julia Ring, Sabrina Schröder, Christoph Magnes, Lucia Antonacci, Heike Fussi, Luiza Deszcz, Regina Hartl, Elisabeth Schraml, Alfredo Criollo, Evgenia Megalou, Daniela Weiskopf, Peter Laun, Gino Heeren, Michael Breitenbach, Beatrix Grubeck-Loebenstein, Eva Herker, Birthe Fahrenkrog, Kai-Uwe Fröhlich, Frank Sinner, Nektarios Tavernarakis, Nadege Minois, Guido Kroemer, Frank Madeo. Induction of autophagy by spermidine promotes longevity.

Nature Cell Biology, Oct. 2009, accepted

Kienesberger PC, Lee D, Pulinilkunnil T, Brenner DS, Cai L, Magnes C, Koefeler

HC, Streith IE, Rechberger GN, Haemmerle G, Flier JS, Zechner R, Kim YB, Kershaw

EE. Adipose triglyceride lipase (ATGL) deficiency causes tissue-specific changes

in insulin signaling.

J Biol Chem. 2009 Aug 31. [Epub ahead of print]

Magnes C, Suppan M, Pieber TR, Moustafa T, Trauner M, Haemmerle G, Sinner FM.

Validated comprehensive analytical method for quantification of coenzyme A

activated compounds in biological tissues by online solid-phase extraction

LC/MS/MS.

Anal Chem. 2008 Aug 1;80(15):5736-42.

Sinner FM, Gatschelhofer C, Mautner A, Magnes C, Buchmeiser MR, Pieber TR.

Ring-opening metathesis polymerization-derived monolithic capillary columns for

high-performance liquid chromatography. Downscaling and application in medical

research.

J Chromatogr A. 2008 May 16;1191(1-2):274-81.

Horvath K, Bock G, Regittnig W, Bodenlenz M, Wutte A, Plank J, Magnes C,

Sinner F, Fürst-Recktenwald S, Theobald K, Pieber TR. Insulin glulisine, insulin

lispro and regular human insulin show comparable end-organ metabolic effects: an

exploratory study.

Diabetes Obes Metab. 2008 Jun;10(6):484-91.

Wutte A, Plank J, Bodenlenz M, Magnes C, Regittnig W, Sinner F, Rønn B,

Zdravkovic M, Pieber TR. Proportional dose-response relationship and lower

within-patient variability of insulin detemir and NPH insulin in subjects with

type 1 diabetes mellitus.

Exp Clin Endocrinol Diabetes. 2007 Jul;115(7):461-7.

Pickl KE, Magnes C, Bodenlenz M, Pieber TR, Sinner FM. Rapid online-SPE-MS/MS

method for ketoprofen determination in dermal interstitial fluid samples from

rats obtained by microdialysis or open-flow microperfusion.

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):432-9.

Gatschelhofer C, Magnes C, Pieber TR, Buchmeiser MR, Sinner FM. Evaluation of

ring-opening metathesis polymerization (ROMP)-derived monolithic capillary high

performance liquid chromatography columns. J Chromatogr A. 2005 Oct

7;1090(1-2):81-9. PubMed PMID: 16196136.

Magnes C, Sinner FM, Regittnig W, Pieber TR. LC/MS/MS method for quantitative

determination of long-chain fatty acyl-CoAs.

Anal Chem. 2005 May 1;77(9):2889-94.

Plank J, Bodenlenz M, Sinner F, Magnes C, Görzer E, Regittnig W, Endahl LA,

Draeger E, Zdravkovic M, Pieber TR. A double-blind, randomized, dose-response

study investigating the pharmacodynamic and pharmacokinetic properties of the

long-acting insulin analog detemir.

Diabetes Care. 2005 May;28(5):1107-12.

Poster

Christina Gatschelhofer, Christoph Magnes, Michael R. Buchmeiser, Franz Stelzer, Thomas R. Pieber, Frank M. Sinner:

Monolithic Stationary Phases Prepared by Ring-Opening Metathesis Polymerisation: Influence of Different Polymerisation Parameters on Separation Performance

7^th Symposium on Instrumental Analysis, 21. - 24. 09. 2003, Pécs, Hungary

Christoph Magnes, Christina Gatschelhofer, Thomas R. Pieber, Frank M. Sinner:

Separation Performance of Different Stationary Phases for Human Insulin and Insulin Analogues – Quantification Limits by LC/MS/MS

7^th Symposium on Instrumental Analysis, 21. - 24. 09. 2003, Pécs, Hungary

Christoph Magnes, Christina Gatschelhofer, Karin Pickl, Thomas R. Pieber, Frank M. Sinner:

Strategies for Quantification of Insulin and Insulin Analogues by LC MS/MS

52^nd ASMS Conference on Mass Spectrometry and Allied Topics, 23. - 27. 05. 2004, Nashville, TE, USA

Christina Gatschelhofer, Christoph Magnes, Thomas R. Pieber, Frank M. Sinner:

Monolithic Stationary Phases Prepared by Ring-Opening Metathesis Polymerisation for cap-HPLC

1^st Monolithic Summer School, 06. - 09. 06. 2004, Portoroz, Slovenia

Christina Gatschelhofer, Christoph Magnes, Agnes Mautner, Michael R. Buchmeiser, Thomas R. Pieber, Frank M. Sinner:

Downscaling of Monolithic Capillary Columns Prepared by Ring-Opening Metathesis Polymerization

29^th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 26. - 30. 06. 2005, Stockholm, Sweden

Christina Gatschelhofer, Agnes Mautner, Michael R. Buchmeiser, Thomas R. Pieber, Frank M. Sinner:

ROMP-Based Monolithic Capillary Columns for Ion-Exchange Liquid Chromatography

21^st International Symposium on Microscale Bioseparations, 14. - 18. 01. 2007, Vancouver, Canada

Christina Gatschelhofer, Agnes Mautner, Christoph Magnes, Michael R. Buchmeiser, Thomas R. Pieber, Frank M. Sinner:

ROMP-Based Monolithic Capillary Columns as ESI-Interface for Fast and Sensitive LC-MS Analysis

55^th ASMS Conference on Mass Spectrometry and Allied Topics, 03. - 07. 06. 2007, Indianapolis, IN, USA

Christoph Magnes, Frank M. Sinner, Lukas Schaupp, Betina Mayr, Michael R. Buchmeiser, Thomas R. Pieber.

Nano-Monolithic Stationary Phases Prepared by Ring-Opening Methatesis

7th International Symposium Advances in Analytical Separation Science  Chromatography and Electrophoresis, 3-5 Juni, 2002, Pörtschach/Wörthersee (Austria) –  POSTER AWARD

Christoph Magnes, Frank M. Sinner, Lukas Schaupp, Michael R. Buchmeiser, Thomas R. Pieber

Monlithic Stationary Phases Prepared by Ring Opening Metathesis Polymerisation used for nano-HPLC/MS: Applications for Clinical Research

22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides, 10 –13. November 2002, Heidelberg (Germany)

Christoph Magnes, Frank M. Sinner, Lukas Schaupp, Thomas R. Pieber

Neue Möglichkeiten in der Diabetesforschung durch Einsatz neuer analytischer Kopplungstechniken

30. Jahrestagung der österreichischen Diabetesgesellschaft, 28.11. - 30.11.2002, Graz (Austria),

Karin Pickl, Christoph Magnes, Ernst Lankmayr, Thomas R. Pieber, Frank M. Sinner

“Influence of Different Sample Preparation Techniques on the Simultaneous Quantification of Different Insulins in Serum using cap-HPLC/MS²

29^th Symposium on High Performance Liquid Phase Separations and Related Topics, 26 – 30th June, 2005, Stockholm, Sweden.

Karin Pickl, Christoph Magnes, Ernst Lankmayr, Thomas R. Pieber, Frank M. Sinner

Optimization of Sample Preparation for Simultaneous Measurement of Different Insulins and Insulin Analogues in Serum using cap-HPLC/MS²

8^th Symposium on Instrumental Analysis, 25 – 28th September, 2005, Graz, Austria.

Karin Pickl, Christoph Magnes, Manfred Bodenlenz, Günter Fauler, Thomas R. Pieber, Frank M. Sinner

Comparison of fenoprofen and deuterated ketoprofen as internal standards for ketoprofen analysis in microdialysis and open-flow microperfusion samples

54^nd ASMS Conference on Mass Spectrometry and Allied Topics, May 29 – June 1st, 2006, Seattle (Washington, USA).

Karin Pickl, Christoph Magnes, Thomas R. Pieber, Frank M. Sinner

New strategy for insulin and insulin analogs quantification - MRM detection for insulin B chain

55^nd ASMS Conference on Mass Spectrometry and Allied Topics, June 3rd – 7th, 2007, Indianapolis (Indiana, USA)

Karin Pickl, Christoph Magnes, Thomas R. Pieber, Hans-Georg Frank, Christoph Schmitz, Frank M. Sinner

Strategies for sensitive peptide detection in brain homogenate using LC/MS for the purpose of blood-brain-delivery testing

56nd ASMS Conference on Mass Spectrometry and Allied Topics, June 1rd – 5th, 2008, Denver (Colorado, USA)

Karin Pickl, Olivia Schnitzer, Christoph Magnes, Thomas R. Pieber, Frank M. Sinner

 “Sensitive Insulin Analysis in Interstitial Fluid: Influence of Mobile Phase Additives o Insulin B chain Loading Efficiency in cap-HPLC/MS²”

9th Symposium on Instrumental Analysis, 29th June – 2nd July, 2008, Pecs, Hungary

Karin Pickl, Christoph Magnes, Thomas R. Pieber, Frank M. Sinner

Ultrafiltration as a purification step in sample preparation for LC/MS² analysis of insulin in serum”

34th International Symposium on High-Performance Liquid Phase Separations and Related Techniques, 28th June – 2nd July, 2009, Dresden, Germany

C. Magnes, W. Regittnig, T. R. Pieber, O. Tehlivets, S. Kohlwein, F. M. Sinner

A Sensitive HPLC/MS/MS Method for the Determination of Short- and Long Chain Fatty Acyl-CoAs in Biological Tissues

29 th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2005) to be held June 26-30, 2005, in Stockholm, Sweden. 

Christoph Magnes; Maria Suppan; Thomas Pieber; Guenter Haemmerle; Frank Michael Sinner

Validated Analytical Method for Quantification and Identification of Coenzyme-A Activated Compounds in Biological Tissues by online SPE-LC/MS/MS

56nd ASMS Conference on Mass Spectrometry and Allied Topics, June 1rd – 5th, 2008, Denver (Colorado, USA)

Christoph Magnes; Maria Suppan; Petra Kienesberger; Tarek Moustafa; Thomas Pieber; Frank Michael Sinner

Acyl CoA-Profiling in Biological Tissues using Online SPE-LC-FTMS (Orbitrap)

57th ASMS Conference on Mass Spectrometry and Allied Topics, 31.05. - 04. 06. 2009, Philadelphia, PA, USA

Maria Suppan, Christoph Magnes, Barbara Daum, Peter Stadler, Thomas R. Pieber, Frank M. Sinner

A validated analytical method for the determination of L-cystine, N-acetyl-L-cysteine, L-cysteine and rearrangement products in amino acid solutions by LC/MS/MS

9th Symposium on Instrumental Analysis, 29th June – 2nd July, 2008, Pecs, Hungary

• A double-blind, randomized, dose-response study investigating the pharmacodynamic and pharmacokinetic properties of the long-acting insulin analog detemir.
• A new strategy for the sensitive and simultaneous analysis of human insulin and insulin analogues using LC/MS² in combination with a tailored sample clean-up procedure
• A novel minimally-invasive approach for investigations into skin barrier function and percutaneous drug penetration
• A Sensitive HPLC/MS/MS Method for the Determination of Short- and Long Chain Fatty Acyl-CoAs in Biological Tissues
• A Validated Analytical Method for Quantification and Identification of Coenzyme-A Activated Compounds in Tissue Samples by online SPE-LC/MS/MS
• A validated analytical method for the dermination of L-Cystine, N-Acetyl-L-Cysteine, L-Cysteine and rearrangement products in amino acid solutions by LC/MS/MS
• Absolute/relative bioavailability and metabolism of dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (tetraenes) after intravenous and oral single doses to rats.
• Adipose Triglyceride Lipase Deficiency Causes Tissue-specific Changes in Insulin Signaling
• Dermal PK/PD of a lipophilic topical drug in psoriatic patients by continuous intradermal membrane-free sampling
• Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase
• Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase.
• Evaluation of ring-opening metathesis polymerization (ROMP)-derived monolithic capillary high performance liquid chromatography columns.
• Functionalization of ROMP-derived monolithic capillary columns and their chromatographic properties
• Identification of coenzyme A activated substances by accurate mass determination using ESI triple quadrupole mass spectrometry
• Induction of autophagy by spermidine promotes Longevity
• Induction of autophagy by spermidine promotes longevity.
• Insulin glulisine, insulin lispro and regular human insulin show comparable end-organ metabolic effects: an exploratory study.
• Insulin glulisine, insulin lispro and regular human insulin show comparable end-organ metabolic effects: an exploratory study.
• LC/MS/MS method for quantitative determination of long-chain fatty acyl-CoAs.
• Monolithic Stationary Phases Prepared by Ring-Opening Metathesis Polymerisation: Influence of Different Polymerisation Parameters on Separation Performance
• Mögliche Hepatoselektivität von Insulin Detemir im Vergleich zu NPH Insulin in Patienten mit Typ-1-Diabetes
• New strategy for insulin andinsulin analogs quantification – MRM detection for insulin B chain
• Norbornene-based monolithic capillary columns for clinical research
• Novel catheters for in vivo research and pharmeceutical trials providing direct access to extracellular space of target tissues
• Online SPE-LC/FTMS (Orbitrap) Technique for Metabolite Profiling (Acyl CoAS)
• Profiling of Acyl CoAs in Biological Tissues using Online SPE-LC-FTMS (Orbitrap)
• Proportional dose-response relationship and lower within-patient variability of insulin detemir and NPH insulin in subjects with type 1 diabetes mellitus.
• Proportional dose-response relationship and lower within-patient variability of insulin detemir and NPH insulin in subjects with type 1 diabetes mellitus.
• Rapid online-SPE-MS/MS method for ketoprofen determination in dermal interstitial fluid samples from rats obtained by microdialysis or open-flow microperfusion.
• Rapid online-SPE-MS/MS method for ketoprofen determination in dermal interstitial fluid samples from rats obtained by microdialysis or open-flow microperfusion.
• Ring-Opening Metathesis Polymerisation-derived Capillary Monoliths: Versatile Analytical Tools for Modern Biomedical Research
• Ring-Opening Metathesis Polymerisation-derived Capillary Monoltihs: Versatile Analytical Tools for Modern Biomedical Research
• Ring-opening metathesis polymerization-derived monolithic capillary columns for high-performance liquid chromatography. Downscaling and application in medical research
• Ring-opening metathesis polymerization-derived monolithic capillary columns for high-performance liquid chromatography: downscaling and application in medical research.
• ROMP-based monolithic capillary columns as ESI-Interface for fast and sensitive LC-MS analysis
• Romp-based monolithic capillary columns for ion-exchange liquid chromatography
• Senstive insulin analysis in interstitial fluid: Influence of mobile phase additives on insulin B chain loading efficiency in CAP-HPLC/MS
• Spermidine promotes mating and fertilization efficiency in model organisms.
• Strategies for Quantification of Insulin and Insulin Analogues by LC MS/MS
• Strategies for sensitive peptide detection in brain homogenate using LC/MS for the purpose of blood-brain-delivery testing
• The interstitium: an analytical challenge
• The propeptide of yeast cathepsin D inhibits programmed necrosis.
• Ultrafiltration as a purification step in sample preparation for LC/MS² analysis of insulin in serum
• Validated comprehensive analytical method for quantification of coenzyme A activated compounds in biological tissues by online solid-phase extraction LC/MS/MS
• Validated comprehensive analytical method for quantification of coenzyme A activated compounds in biological tissues by online solid-phase extraction LC/MS/MS.
• Validated Comprehensive Analytical Method for Quantification of Coenzyme-A Activated Compounds in Biological Tissues by Triple Quadrupol LC/MS/MS