Quantification of metabolites
Complete quantification of metabolites using isotope-labeled internal standards.
Profiling classes of metabolites
- Coenzyme A activated substances isolated from tissues, cell and yeast cultures
- Polyamines from serum / plasma / tissue (human / animal) samples
- Phospholipids, triglycerides
Profiling small molecules in biological samples using chromatography coupled with high-resolution mass spectrometry (Orbitrap). Alterations in patterns of small molecules are examined using sophisticated statistical methods that have tailored for biomarker identification.
Profiling classes of metabolites - targeted / untargeted metabolomics
LC coupled to high-resolution mass spectrometry based on Orbitrap technology allows the identification of metabolites in fingerprints on the basis of their exact molecular mass and retention time. This technology enables the user to study small molecule profiles while simultaneously targeting specific substances and substance classes.
Application: hypothesis-driven biomarker discovery, characterization of pharmaceuticals from natural sources (tea, plant extracts), metabolic research.
Optimized methods for quenching cellular metabolism
Specific metabolites resulting from energy metabolism are, in turn, metabolized at a high conversion speed. In order to carry out investigations on these metabolites, the background enzymatic reactions must be stopped (quenched) quickly and exactly at the desired time point. The metabolite concentrations are then preserved in the sample. This can be achieved by optimizing the methods for quenching cellular metabolism.