Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood

Publication from Materials
Hybridelektronik und Strukturierung

Anupam A.K. Das, Jevan Medlock, He Liang, Dieter Nees, David J. Allsup, Leigh A. Madden, Vesselin N. Paunov

Journal of Materials Chemistry B Issue 22, 5/2019


We report a large scale preparation of bioimprints of layers of cultured human leukemic HL60 cells which can perform cell shape and size recognition from a mixture with peripheral blood mononuclear cells (PBMCs). We demonstrate that the bioimprint-cell attraction combined with surface modification and flow rate control allows depletion of the HL60 cells from peripheral blood which can be used for development of alternative therapies of acute myeloid leukaemia (AML).