Models and Technologies

Models for examining or testing of wound healing that are based on human components, offer the advantage that the results of effectiveness studies can be implemented more directly in clinical application.

On the one hand, experiments can be carried out in three-dimensional in vitro models, which consist of at least one dermal and epidermal part. On the other hand, it is possible to examine the cutaneous reactions in recent explants (ex vivo).

For the testing and evaluation of novel substances various skin models are available, starting with basic analysis of 2D monocultures of keratinocytes or fibroblasts, to co-culture models and complex 3D models.

in vitro model
Photo credit: JOANNEUM RESEARCH/Schwarzl

Using fresh human skin explants, which are removed in elective surgical interventions, experiments on early cutaneous reactions of the skin after injuries like e.g. burns can be realized. Additionally, also the effects of certain wound dressings can be investigated using human skin explants.

ex vivo model
Photo credit: JOANNEUM RESEARCH/Schwarzl


  • Hofmann E, Fink J, Eberl A, Prugger EM, Kolb D, Luze H, Schwingenschuh S, Birngruber T, Magnes C, Mautner SI, Kamolz LP, Kotzbeck P. A Novel Human Ex Vivo Skin Model to Study Early Local Responses to Burn Injuries. Sci Rep. 2020. Accepted

  • Holzer JCJ, Tiffner K, Kainz S, Reisenegger P, Bernardelli de Mattos I, Funk M, Lemarchand T, Laaff H, Bal A, Birngruber T, Kotzbeck P, Kamolz LP. A novel human ex-vivo burn model and the local cooling effect of a bacterial nanocellulose-based wound dressing. Burns. 2020 Dec;46(8):1924-1932. doi: 10.1016/j.burns.2020.06.024. Epub 2020 Jul 3.